Isopropyl β-d-1-thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator. WebApr 15, 2024 · The molecular weight of the recombinant alkaline protease, as determined by SDS-PAGE and the Mass Spectrometer (MALDI-TOF), was 34 kDa. ... The flasks were supplemented with 0.5 mM, 1.0 mM, and 2.0 mM IPTG (isopropyl-β-D-thiogalactopyranoside), respectively, to investigate the induction effect. The culture flasks …
Practical protocols for production of very high yields of …
WebOct 1, 2024 · The molecular weight of IPTG (CAS 367-93-1) is 238.3 g/mol which means you need to dissolve 238.3 grams of IPTG in water to prepare 1 L of 1M solution. Requirements: Reagents ♦ IPTG powder ♦ Deionized / Milli-Q water Equipment and disposables ♦ Falcon® Conical Tubes, 15 mL Composition: 1M IPTG in water Objective: WebApr 4, 2024 · At that time, the culture was cooled down on ice for 30 min, and 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) was added. The expression of the recombinant C4C5 was induced overnight at 16 °C with shaking at 225 rpm. ... MRW or mean residue weight is M/(N–1), where M is the molecular weight in g/mol, and N is the number of … small bacterial overgrowth diagnosis
Molecular Weight of IPTG (C9H18O5S) - Mol-Instincts
Web14 hours ago · For fusion protein production, the DNA was transformed into BL21(DE3) competent cells for high protein expression and induced mid-log phase with 0.4 mM IPTG 16–20 h at 22 °C. WebFormula: C9H18O5S Formula Weight: 238.31 CAS Number: 367-93-1 Synonym(s): isopropyl-beta-D-Thiogalactopyranoside Shop MilliporeSigma OmniPur® IPTG at Thomas Scientific, your trusted partner in Science. WebTitle: IPTG Molecular Formula: C9H18O5S Molecular Weight: 238.30 Percent Composition: C 45.36%, H 7.61%, O 33.57%, S 13.45% Standard InChI: InChI=1S/C9H18O5S/c1-4 (2)15-9-8 (13)7 (12)6 (11)5 (3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1 Standard InChIKey: BPHPUYQFMNQIOC-NXRLNHOXSA-N Last Revised: 2013 © Royal Society of Chemistry solifer s6+