WebbA His-tag (polyhistidine tag) consists of at least six histidine residues that are located at the N- or C-terminus of recombinant proteins. It is commonly used for affinity purification and protein binding experiments. The recombinant protein has only 6 histidine residues as a tag. Is this sufficient to be detected by your antibody? WebbRecognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags) Application Info. WB (ECL): 1:1000~3000 (incubate for one hour at room temperature) IP: 1:50~200. IS: 1:500~2000.

His标签抗体在WB和ELISA中的应用 - 知乎

WebbHRP anti-His Tag Antibody. A 96 well plate is coated with 100 µL of a His-tagged recombinant protein at 100 ng/mL. The coated protein is detected with HRP-conjugated … WebbThe antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells. Expand Description Specifications Description: Anti-6X HIS EPITOPE TAG (MOUSE) Monoclonal Antibody Peroxidase Conjugated - 200-303-382 aquaman black manta submarine

Why is my his-tagged protein not detected in my western …

Webb筛选器： WB IP 使用 His-Tag (27E8) Mouse mAb，对对照裂解物（泳道 1）或 C 末端有 His 标签的蛋白质（泳道 2）进行免疫沉淀，随后采用 His-Tag Polyclonal Antibody #2365 进行蛋白质印迹分析。 使用 His-Tag … WebbHis-tag is one of the most commonly used tags. It is a series of six to nine histidine residues fused to either the amino or carboxyl terminus of a recombinant protein. His … Webb10001-0-AP targets 6*his,His tag in WB, IP, IHC, IF, ELISA applications and shows reactivity with recombinant protein samples. PBS with 0.02% sodium azide and 50% … aquaman bionik m